Preliminary effects of olive mill wastewater on the glucose absorption in mice

N. Senani-Oularbi, D. El benna, J.C. Marie, F. Moulti-Mati


Abstract: Olive mill wastewater (OMWW) is the liquid by-product generated during olive oil production.  The aim of this study is to investigate the effect of OMWW on intestinal glucose absorption in mice, notably for treating diabetes. Oral glucose tolerance test (OGTT) is a medical test in which glucose is given and blood samples taken afterward to determine how quickly it is cleared from the blood. This test was performed in Male C57BL/6J mice weighing 20–25 g.  Gavages of mice with glucose were performed after a 16-h fast using a D-glucose solution (1 g/Kg) without or with OMWW, in dose range of 0.5– 4 g/kg of body weight. The bleeds were further taken at 15, 30, 60, and 120 min after oral glucose administration. These experiments were performed at least with 6 individual animals. Oral glucose tolerance test carried out in mice showed that oral administration of OMWW decreased glycemia at all tested concentration, which is significant at concentration of 4g/Kg of body weight. These results suggest OMWW have potentially hypoglycemic effect in mice.

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Cassano, A. ; Conidi, C. ; Giorno, L. ; Drioli, E. Fractionation of olive mill wastewaters by membrane separation techniques. Journal of Hazardous Materials (248–249) (2013) 185–193.

Eroğlu, E. ; Eroğlu, I., Gündüz, U.; Türker, L.; Yücel, M. Biological hydrogen production from olive mill wastewater with two-stage processes. International Journal of Hydrogen Energy (31) (2006) 1527–1535.

Dermeche, S.; Nadour, M., Larroche, C., Moulti-Mati, F., Michaud, P. Olive mill wastes: biochemical characterizations and valorization strategies. Process Biochemistry 48 (2013) 1532–1552.

Hamden, K.; Allouche, N.; Damak, M; Elfeki, A. Hypoglycemic and antioxidant effects of phenolic extracts and purified hydroxytyrosol from olive mill waste in vitro and in rats. Chemico-biological Interactions 180 (3) (2009) 421–432.

Obeid, H.K.; Allen, M.S.; Bedgood, D.R.; Prenzied, P.D., Robards, K.; Stockmann, R. Bioactivity and analysis of biophenols recovered from olive mill waste. Journal of Agricultural and Food Chemistry 53 (2005) 823–837.

Casalino, E. ; Calzaretti, G. ; Sblano, C. ; Landriscina, V. ; Tecce, M.F. ; Landriscina, C. Antioxidant effect of hydroxytyrosol (dpe) and mn2+ in liver of cadmium intoxicated rats. Comparative biochemistry and physiology part c.133 (2002) 625–632.

Abdelmoaty, M.A.; Ibrahim, M.A.; Ahmed, N.S.; Abdelaziz, M.A. Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats. Indian Journal of Clinical Biochemistry 25(2010) 188–92.

Rajiv Gandhi G.; Sasikumar P. Antidiabetic effect of merremiae marginata burm. F. In streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine 2 (2012) 281-286.

Abo, K.A.; Fred-Jaiyesimi, A.A.; Jaiyesimi, A.E. Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in south western nigeria. Journal of Ethnopharmacology 115 (2008) 67–71.

Vishwakarma, S.L.; Rakesh, S.; Rajani, M.; Goyal, R.K. Evaluation of effect of aqueous extract of enicostemmalittorale blume. In streptozotocin induced type 1 diabetic rats. Indian Journal of Experimental Biology 48 (2010) 26-30.

Syamsudin. Standardization of extract of leucaenaleucocephala (lmk) de wit seeds by α- glucosidase inhibitor. International Journal of Phytomedicine 2 (2010) 430-435.

El-abbassi, A.; Hajar, K.; Abdellatif, H. Phenolic profile and antioxidant activities of olive mill wastewater. Food Chemistry 132 (2012) 406–412.

Bradford,; MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1976) 248-254.

Knight J.A.; Shauna A,; James, M. R.(1972), Chemical basis of the sulfo-phospho-vanillin reactionfor estimating total serum lipids. Clinical Chemistry, 18(3), 199-202.

Dubois, M.; Gilles, K.A; Hamilton, J. K. ; Rebers, P. A. ; Smith, F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28 (3) (1956) 350-356.

Miller, G. L. Use of dinitrosaiicyiic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3) (1959) 426-428.

De marco, E. ; Savarese, M. ; Paduano, A. ; Sacchi R. Characterization and fractionation of phenolic compounds extracted from olive oil mill wastewaters. Analytical Nutritional and Clinical Methods. Food chemistry (2007) 858–867.

Folin, O., & Ciocalteau, V. On tyrosine and tryptophan determination in protein. Journal of Biological Chemistry 73(1927) 627–650.

Belharbi, K. R. ; Lettéron, P. ; Chedid, P. ; Nazaret C, Ducroc, R. ; Marie, J-C. Resistin-like molecule_ inhibits sglt-1 activity and enhances glut2-dependent jejunal glucose transport. Diabetes 58 (2009) 2032–2038.

Eroglu, E.; Gunduz, U.; Yucel, M.;Turker, L.; Eroglu, I. Photobiological hydrogen production by using olive mill wastewater as a sole substrate source. Internantional Journal of Hydrogen energy 29(2) (2004) 163–71.

Hamdi, M. Toxicity and biodegradability of olive mill wastewaters in batch anaerobic digestion. Applied biochemistry and biotechnology 37(1992) 155–63.

Eroglu, E.; Eroglu, I.; Gunduz, U.; Yucel, M. Treatment of olive mill wastewater by different physicochemical methods and the utilization of their liquid effluents for biological hydrogen production. Biomass and Bioenergy 334 (2009) 701–5.

Vierhuis, E.; Korver, M.; Schols, H. A. ; Voragen, A. G. Structural characteristics of pectic polysaccharides from olive fruit (olea europaea cv moraiolo) in relation to processing for oil extraction. Carbohydrate Polymers 51(2) (2003) 135-148.

Leney, S. E, Tavaré, J.M. The molecular basis of insulinstimulated glucose uptake: signaling, trafficking and potential drug targets. Journal of Endocrinology 203 (2009) 1-18.

Wu, C.; Okar, D.A. ; Kang, J.; Lange, A.J . Reduction of hepaticglucose production as a therapeutic target in the treatment of diabetes. Current drug targets- immune endocrine metabolic disorders 5 (2005) 51-59.

Mu, L.H. ; Huang, Z.X, Liu, P.; Hu,Y.; Gao, Y. Acute and subchronic oral toxicity assessment of the herbal formula kai-xin-san. Journal of Ethnopharmacology 138 (2011) 351-357.

Nesseris, G. K.; Stasinakis, A. S. Investigation of municipal and olive mill wastewater co-treatment in activated sludge–powdered activated carbon (as-pac) systems. Journal of Chemical Technology and Biotechnology 87 (4) (2012) 540–545.

Filidei, S. ; Masciandaro, G. ; Ceccanti, B. Anaerobic digestion of olive oil mill effluents: evaluation of wastewater organic load and phytotoxicity reduction. Water, Air, & Soil Pollution 145(1) (2003) 79-94.

Hwang J.T.; Kwon D.Y.; Yoon, S.H. Amp-activated protein kinase: a potential target for the diseases prevention by natural occurring polyphenols. New Biotechnology 26 (2009) 17-22.

Nadour, M. ; Michaud, P. ; Moulti-Mati, F. Antioxidant activities of polyphenols extracted from olive (olea europaea) of chamlal variety. Appl Biochem Biotechnol 167 (6) (2012) 1802 -1810.

Dudley, J.I.; Lekli, I.; Mukherjee, S.; Das, M. ; Bertelli, A.A. ; As, D.K . Does white wine qualify for french paradox? Comparison of the cardioprotective effects of red and white wines and their constituents: resveratrol, tyrosol, and hydroxytyrosol. Journal of Agricultural and Food Chemistry 56 (2008) 9362–9373.

Zhang, X.; Jiang, L.; Geng, C.; Yoshimura, H.; Zhong, L. Inhibition of acrylamide genotoxicity in human liver-derived hepg 2 cells by the antioxidant hydroxytyrosol. Chemico-Biological Interactions 176 (2008) 173–178.

Deiana, M. ; Incani, A. ; Rosa, A. ; Corona, G. ; Atzeri, A., Loru, D. ; Melis, M.P. ; Dessì, M.A. Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on h2o2 induced lipid peroxidation in renal tubular epithelial cells. Food and Chemical Toxicology 46 (2008) 2984–2990.


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