Utilisation of agro alimentary wastes for bio surfactants production by a thermophilic bacterial strain novelty isolated from an Algerian crude oil contaminated soil

K. Mokdad, F.Z. Mesbaiah, K. Eddouaouda, A. Badis

Abstract


Abstract: In this work we have undertaken a study based on the production and characterization of biosurfactants from a thermophilic bacterial strain bacteria isolated from soil contaminated with petroleum hydrocarbons. The results indicate a reduction in the surface tension (32 mN /m) by the strain 1J. The influence of various parameters (carbon source, nitrogen source, pH, salinity) on the emulsifying activity and the surface tension reduction revealed that the olive oil mill effluent (0.5%) and ammonium chloride present the best carbon and nitrogen sources for biosurfactant production by this strain. The maximum of biosurfactant production was obtained at near neutral pH and a salinity of 2%. The biosurfactant produced by our strain improved a resistance to extreme conditions of temperature (4 to 121°C), pH (2-12) and salinity (up to 250 g / l). These interesting characteristics of our local biosurfactant find numerous applications in various food areas.


Full Text:

PDF

References


Kapadia S.G., Yagnik B.N. Current trend and potential for microbial biosurfactants. Asian J. Exp. Biol.Sci. 2013;4:1–8.

Eddouaouda kamal, Sami Mnif, Abdelmalek Badis, Sonia Ben Younes, Slim Cherif, Samira Ferhat, Najla Mhiri, Mohamed Chamkha1 and Sami Sayadi.. "Characterization of a novel biosurfactant produced by Staphylococcus sp. strain 1E with potential application on bioremediation of hydrocarbons contaminated sites". Journal of Basic Microbiology, 51, pp. (2012) 1 – 11.

Jaeger KE, Dijkstra BW, Reetz MT., "Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases". Annual Rev Microbiol, 53, (1999), 315-351.

Rodrigues, L., Moldes, A., Teixeira, J., & Oliveira, R., "Kinetic study of fermentative biosurfactant production by Lactobacillus strains". Biochemical Engineering Journal, 28, (2006),109-116.

Abouseoud, M., Maachi, R., Amrane, A., Boudergua, S. et Nabi, I., “Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens”. Desalin, V. 223, n° 1 et 3, (March 2008), 143-151.

Sarubbo LA, Luna JM, Campos-Takaki GM., "Production and stability studies of the bioemulsifiers obtained from a new strain of Candida glabrata UCP 1002". Electronic Journal of Biotechnology, 9, (2006), 400-406.

Soberón-Chávez G, Maier RM., “Biosurfactants: a General Overview. In: Soberón-Chávez G, editor. Biosurfactants. Springer-Verlag; Berlin, Germany“, (2011), pp. 1–11.

Mercade ME, Monleon L, deAndres C, Rodon I, Martinez E, Espuny MJ, Manresa A (1996) Screening and selection of surfactant- producing bacteria from waste lubricating oil. J Appl Bacteriol 81:161–168.

Dubey K, Juwarkar A., “Determination of genetic basis for biosurfactant production in distillery and curd whey wastes utilizing Pseudomonas aeruginosa strain BS2“. Indian Journal of Biotechnology, 3, (2004),74-81.

Daniel HJ, Reuss M, Syldatk C., “Production of sophorolipids in high concentration from deproteinized whey and rapeseed oil in a two stage fed batch process using Candida bombicola ATCC 22214 and Cryptococcus curvatus ATCC 20509“. Biotechnology Letters, 20, (1998),115356.

Urai, M., Aizawa, T., Anzai, H., Ogihara, J., Iwabuchi, N., Neilan, B.,Couperwhite, I., Nakajima, M. & Sunairi, M., "Structural analysis of an extracellular polysaccharide produced by a benzenetolerant bacterium, Rhodococcus sp". 33. Carbohydr Res, 341, (2006), 616-623.

Ferhat samira, Sami Mnif, Abdelmalek Badis, Kamel Eddouaouda, Redha Alouaoui, Ahmed Boucherit, Najla Mhirib, Nadji Moulai-Mostefa, Sami Sayadi. “Screening and preliminary characterization of biosurfactants produced by Ochrobactrum sp. 1C and Brevibacterium sp. 7G isolated from hydrocarbon-contaminated soils“. International Biodeterioration & Biodegradation, 65, (2011), pp.1182 – 88.

Lee K, Hwang SH, Ha S, Jang JH, Lim DJ, Kong JY., “Rhamnolipid production in batch and fed- batch fermentation using Pseudomonas aeruginosa BYK-2 KCTC 18012P“. Biotechnol Biopro Eng, 9, (2004), 267–273.

Cameotra SS, Makkar RS., "Synthesis of biosurfactants in extreme conditions". Appl Microbiol Biotechnol, 50, (1998), 520–529

Deleu M, Paquot M., "From renewable vegetables resources to microorganisms: new trends in surfactants". Comptes Rendus Chimie, 7, (2004), 641–646.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License. Copyright UMMB © 2020 University M'hamed Bougara - Boumerdes. Independance AV., Boumerdes, 35000 Algeria, Tel/Fax: +213 24 91 14 98/ +213 2491-29-51