Protective effect of olive mill wastewater against lipid metabolism perturbation induced in cafeteria diet fed rats

D. Messaoudi, Z. Bouallagui, A. Henab, F. Moulti-Mati


Abstract: The biological activities of phenolic compounds from olive mill wastewater (OMWW) have been extensively studied. However, the bioactivity of raw OMWW has not been yet well demonstrated in vivo. The objective of this study was to evaluate the effect of oral administration of OMWW on cafeteria diet induced metabolic disorders in rats. Our results showed that within a 13-week treatment period, the cafeteria diet induced an increase in glucose and triglycerides levels. The levels of glucose, triglycerides and the total cholesterol were significantly decreased after supplementation with OMWW (p < 0.05). To evaluate the toxicity effect of OMWW, the results concerning the blood parameters of the renal and liver function (urea, creatinine, uric acid, bilirubin, total protein and transaminases) showed that there are not difference between the control and the OMWW supplementation groups. The liver histology from rats fed with cafeteria diet showed a steatosis which is characterized by lipid droplet accumulation in the hepatocytes cytoplasm. In OMWW supplemented groups, the liver tissue showed a structure similar to the control group.  HPLC analysis of OMWW reveled the major presence of a phenolic acid wich is hydroxytyrosol. In conclusion, our study suggests that OMWW brought real health benefits by protecting liver tissue and modulating some biological parameters such as lipid parameters.

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