Characterization and valorization of olive pomace for production of cellulase from Trichoderma reesei RUT C30 in solid-state fermentation

M. Boutiche, F. Sahir-Halouane, L. Meziant, I. Boulaouad, F. Saci, S. Fiala, A. Bekrar, R. Mesbah, A. Abdessemed

Abstract


Abstract: The aim of the present work is to characterize the olive pomace (physico-chemical and microbiological properties) of four regions known by their high production of olive oil in the north of Algeria and to valorize the lignocellulosic biomass as culture substrate for cellulases production using Trichoderma reesei RUT C-30. The results obtained show that the olive pomace is a favorable environment for the growth of fungi. The load of total aerobic mesophilic flora, yeasts and molds varies from 0.45×106 to 1.36×108 CFU/g DM and from 2×106 to 4.76×107 CFU/g DM, respectively. Physico-chemical parameters of the four regions revealed significant differences (p<0.05). The determination of filter paper activities of enzymatic extracts reveals that olive pomace from Jijel presented the best activity (0.78 U/gds).


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