Evaluation of the quality and composition of lemon (Citrus limon) peel essential oil from an Algerian fruit juice industry

F. Benoudjit, L. Maameri, K. Ouared

Abstract


Abstract: Lemon peel essential oil is used in food, medicines, cosmetics and perfumes, detergents, aromatherapy, pathogen inhibition, and insect control. The present work aimed to investigate the physicochemical characteristics and composition of the essential oil extracted from lemon grown in Algeria. The essential oil was obtained by cold-pressing from lemon peels which lemon barks are waste from an Algerian fruit juice industry. The physicochemical properties of the essential oil were determined. Essential oil was also characterised by FTIR-ATR and by GC-MS. The obtained physicochemical properties results revealed that lemon peel essential oil was a pale yellow aromatic liquid which was miscible in ethanol. It had an approximate pH of 6, a specific gravity of 0.894, a refractive index of 1.475 and an acidic value of 2.10 mg KOH/g. The FTIR spectrum revealed the presence of alkanes, alkenes, aromatic compounds and alcohols. A total of thirty-nine chemical compounds were identified based on GC-MS analysis of the lemon peel essential oil with a variation of percentage.  These compounds are dividing in three different chemical classes (monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpenes and others). The results showed that the monoterpene hydrocarbons are the most abundant ones in the cold pressed lemon peel essential oil which represent about 93% of the total essential oil. The major constituent was α-limonene (~ 65%) and the main aldehydes were geranial (~ 2%) and neral (~ 1%).


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References


Huet, R. Les huilles essentielles d’agrumes. Fruits 46 (1991) 551-576.

Danila, E.; Moldovan, Z.; Popa, M.; Chifiriuc, M. C.; Kaya, A. D.; Kaya, M. A. (2018). Chemical composition, antimicrobial and antibiofilm efficacy of C. limon and L. angustifolia EOs and of their mixtures against Staphylococcus epidermidis clinical strains. Industrial Crops & Products 122 (2018) 483–492.

Hossain, M. A.; AL Harbi1, S. R.; Weli, A. M; Al-Riyami, Q.; Al-Sabahi, J. N. Comparison of chemical constituents and antimicrobial activities of three essential oils from three different brands' clove samples collected from Gulf region. Asian Pacific Journal of Tropical Disease 4 (2014) 262-268.

AL-Jabri, N. N.; Hossain, M. A. Comparative chemical composition and antimicrobial activity study of essential oils from two imported lemon fruits samples against pathogenic bacteria, Beni-Suef University Journal of Basic and Applied Sciences 3 (2014) 247-253.

Baba, E.; Acar, U.; Ontas, C.; Kesbic, O. S.; Yilmaz, S. Evaluation of Citrus limon peels essential oil on growth performance, immune response of Mozambique tilapia Oreochromis mossambicus challenged with Edwardsiella tarda. Aquaculture 465 (2016) 13-18.

Djenane, D. Chemical profile, antibacterial and antioxidant activity of Algerian Citrus essential oils and their application in sardina pilchardus. Foods 4 (2015) 208-228.

Guo, J. J.; Gao, Z. P.; Xia, J. L.; Ritenour, M. A.; Li, G. Y; Shan, Y. Comparative analysis of chemical composition, antimicrobial and antioxidant activity of citrus essential oils from the main cultivated varieties in China. LWT - Food Science and Technology 97 (2018) 825-839.

Simas, D. L. R.; de Amorim, S. H. B. M.; Goulart, F. R. V.; Alviano, S. A.; Alviano, D. S.; da Silva, A. J. R. Citrus species essential oils and their components can inhibit or stimulate fungal growth in fruit. Industrial Crops and Products 98 (2017) 108-115.

Nair, A.; Kurup, R. S. P.; Nair, A. S.; Baby, S. Citrus peels prevent cancer. Phytomedicine 50 (2018) 231-237.

Amorim, J. L.; Simas, D. L. R.; Pinheiro, M. M. G.; Moreno, D. S. A.; Alviano, C. S.; da Silva, A. J. R.; Fernandes, P. D. Anti-inflammatory properties and chemical characterization of the essential oils of four citrus species. Plos One 11 (2016) 1-18.

Gironi,F.; Maschietti, M. Phase equilibrium of the system supercritical carbon dioxide–lemon essential oil : New experimental data and thermodynamic modelling. Journal of Supercritical Fluids 70 (2012) 8-16.

Barth, D.; Chouchi, D.; Perrut, M. Desorption of lemon peel oil by supercritical carbon dioxide: Deterpenation and psoralens elimination. The Journal of Supercritical Fluids 7 (1994) 177-183.

Nguyen, H.; Campi, E. M.; Jackson, W. R.; Patti, A. F. Effect of oxidative deterioration on flavour and aroma components of lemon oil. Food Chemistry 112 (2009) 388-393.

de Rodriguez, G. O.; de Godoy, V. M.; de Colmenares, N. G.; Salas, L. C.; de Ferrer, B. B. Composition of venezuelan lemon essential Citrus limo (L.) Burm.f. Revista de la Facultad de Agronomia del Zulia 15 (1998) 343-349.

Caburian, A. B.; Osi, M. O. Characterization and Evaluation of Antimicrobial Activity of the Essential Oil from the Leaves of Piper betle L. E-International Scientific Research Journal 2 (2010) 2-13.

ISO 1242. Essential oils - Determination of acid value. (1999).

Mehl, F.; Marti, G.; Boccard, J.; Debrus, B.; Merle, P.; Delort, E.; Baroux, L.; Raymo, V.; Velazco, M. I.; Sommer, H.; Wolfender, J. L.; Rudaz, S. Differentiation of lemon essential oil based on volatile and non-volatile fractions with various analytical techniques: a metabolomic approach. Food Chemistry 143 (2014) 325-335.

Van Den Dool, H.; Kratz, P. C. A generalization of the retention index system including linear temperature programmed gas—liquid partition chromatography. Journal of Chromatography A 11 (1963) 463-471.

ISO 855. Oil of lemon [Citruc limon (L.) Burm. F.], obtained by expression. (2003).

Kumar, A. Physico-chemical and natural products investigations of essential oil from the rhizomes of Kaempferia galanga L. Pelagia Der Chemica Sinica 5 (2014) 91-94.

Nijoku, V. I.; Evbuomwan, B. O. analysis and comparative study of essential oil extracted from Nigerian orange, lemon and lime peels. Greener Journal of Chemical Science and Technology 1 (2014) 6-14.

Alam, M. K.; Uddin. M. S. Characterization of oil and lecithin from pioly (Aspidoparia morar) fish. Journal of Innovations in Pharmaceutical and Biological Sciences. 4 (2017) 44-48.

CODEX STAN 19. Norme pour les graisses et les huiles comestibles non visées par des normes individuelles. (1981).

Fabie-Agapin, J. S.; Janagap, S.; Martizano, J.; Ortillo, D.; Azucena-Topor, V. Physico-chemical characterization of essential oil from the peel and leaf of dalanghita (Citrus nobilis). International Journal of Novel Research in Physics Chemistry & Mathematics 2 (2017) 1-13.

Yamauchi, Y.; Saito, M. Fractionation of lemon-peel oil by semi-preparative supercritical fluid chromatography. Journal of Chromarographv. 505 (1990) 231-246.

Mejri, A.; Abderrabba, M.; Mejri, M. Chemical composition of the essential oil of Ruta chalepensis L: Influence of drying, hydro-distillation duration and plant parts. Industrial Crops and Products 32 (2010) 671-673.

Rahimmalek, M.; Tabatabaei, B. I. S.; Etemadi, N.; Goli, S. A. H.; Arzani, A.; Zeinali, H. Essential oil variation among and within six Achillea species transferred from different ecological regions in Iran to the field conditions. Industrial Crops and Products 29 (2009) 348-355.

Hosni, K.; Zahed, N.; Chrif, R.; Abid, I.; Medfei, W.; Kallel, M.; Ben Brahim, N.; Sebei, H. Composition of peel essential oils from four selected Tunisian Citrus species: Evidence for the genotypic influence. Food Chemistry 123 (2010) 1098-1104.

Al-Jabri, N. N.; Hossain, M. A. Chemical composition and antimicrobial potency of locally grown lemon essential oil against selected bacterial strains. Journal of King Saud University – Science 30 (2018) 14-20.


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